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KMID : 0670820100150030124
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Korean Journal of Medical Mycology
2010 Volume.15 No. 3 p.124 ~ p.133
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Antifungal Susceptibility Testing of Dermatophytes Using Etest
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Kang Gyo-Shin
Suh Moo-Kyu
Ha Gyoung-Yim
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Abstract
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Background: It is necessary to perform antifungal susceptibility testing of dermatophytes. Etest (AB Biodisk, Sweden) is a rapid, easy-to-perform in-vitro antifungal susceptibility test.
Objective: The purpose of this study was to investigate the minimal inhibitory concentration (MICs) of dermatophytes isolated from skin using Etest.
Methods: 21 species of dermatophytes (12 strains of T. rubrum, 7 strains of T. mentagrophytes, M. canis and M. gypseum) and two standard strains (Aspergillus flavus KCTC 6905, Aspergillus fumigatus KCTC 6145) were tested MIC endpoints of Etest for itraconazole (ITZ) and amphotericin B (AMB) were read after 72, 96, and 120 hours incubation for each strains on RPMI 1640 agar.
Results: MIC of ITZ was 0.12~0.47 μg/mL on T. rubrum, 0.012~1.0 μg/mL on T. mentagrophytes, 0.012 μg/mL on M. canis, and 0.023 μg/mL on M. gypseum. MIC of AMB was 0.094~0.5 μg/mL on T. rubrum, 0.032~1.0 μg/mL on T. mentagrophytes, 0.19 μg/mL on M. canis, and 0.032 μg/mL on M. gypseum. One strain of T. mentagrophytes isolated from patient with tinea pedis showed ITZ-resistant.
Conclusion: This study showed that Etest represented a simple and efficacious method for antifungal susceptibility testing of dermatophytes.
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KEYWORD
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Etest, Dermatophytes
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